Liquid ordered domains on a GUV
(fluorescent image).
AFM image and line profile of the domains on a supported lipid
bilayer after the GUV rupture.

Formation of Liquid-ordered Domains on a Giant Unilamellar Vesicle

Liquid-ordered (lo) domains (raft-like domains), play an important role in regards to the function and arrangement of membrane proteins. Here, we form lo domains on a giant unilamellar vesicle (GUV). The GUV can be ruptured by increasing the salt concentration in the buffer solution and screening the repulsive electrostatic interaction between the GUV and the substrate. These micron-scale domains on a supported lipid bilayer will help to control membrane proteins in nano-bio devices. (Page 23)